Category Archives: Animal Health Research Institute
Serological Diagnosis of Bovine Brucellosis
Comparative Evaluation of Standard Serological Tests for Diagnosis of Ovine Brucellosis
(Serological Diagnosis of Bovine Brucellosis)
Ashraf, A. Abd El Tawwab 1, Fatma I. El Hofy1, Khoudair M.Ramadan.2, Fatma E. Harb.3
1. Dept. of Bacteriology, Mycology and Immunity. Fac. of Vet. Med., Benha Univ. 2. Dep. of Brucellosis.
Animal Health Research Institute (AHRI. 3. General Organization of Veterinary Services (GOVS)
A B S T R A C T
One hundred blood samples (55 sheep and45 goats) (include 79 females and 21 males)were selected from
farms suffer from brucellosis and examined serologically by Buffered Acidified plate Antigen Test
(BAPAT), Rose Bengal plate Antigen test (RBPAT) , Tube Agglutination test (TAT), Complement Fixation
Test (CFT) and Immunochromatography assay (ICA). The results of BAPAT were (61.81%) and (73.33%),
RBPAT& TAT were (61.81%) and (66.66%) and CFT &ICA were (60%) and (66.66%) in sheep and goats
respectively for all tests. Moreover, the positive reactors in females among sheep and goats were (79.1%),
(76.56%), (81.25%), (80.95%) and (79.36%) using BAPAT, RBPAT, TAT, CFT and ICA respectively. The
sensitivity, specificity and agreement of BAPAT with CFT in sheep and goats were (92.06%, 75.67%, 86%)
respectively. The sensitivity, specificity and agreement of RBPT with CFT in sheep and goats were
(88.88%, 78.37%, 85%) respectively. The sensitivity, specificity and agreement of TAT with CFT in sheep
and goats were (84.12%, 70.27%, 79%) respectively. The sensitivity, specificity and agreement of ICA with
CFT in sheep and goats were (98.41%, 97.29%, 98%) respectively. The sensitivity, specificity of CFT was
100%. In conclusion the ICA proved to be the most accurate, cheapest, rapid and simplest test for diagnosis
of ovine brucellosis.
Keywords: Brucella, serological tests, sheep and goats, Serological Diagnosis of Bovine Brucellosis
Abdelhalim Elshawadfy is an ASCP certified Medical Laboratory Scientist (MLS ASCP), Specialist in Microbiology (SM ASCP), and Molecular Biology Technologist (MB ASCP) with a distinguished career marked by expertise, continuous learning, and a commitment to elevating the standards of clinical laboratory science. As an ASCP Certification Expert and American Board Certification Expert, Abdelhalim Elshawadfy continues to make invaluable contributions to the field, leaving a lasting impact on the medical community.
Microscopic Slides for Practical Part of Poultry Diseases
Dear guys and girls here are the link to download the slides of practical parts of poultry diseases, Specialy Cours 140.
Click here to download the zipped file
of all the slides as taken from
Poultry Department?
To unzip the file click here to download WinRar if you don’t have?
Disclaimer: I have no responsibility for any mistakes or any issue with the slides! plz refer to the Poultry Department for any support if you are obsessive of anything?
Best Regards
Abdelhalim Elhsawadfy
Abdelhalim Elshawadfy is an ASCP certified Medical Laboratory Scientist (MLS ASCP), Specialist in Microbiology (SM ASCP), and Molecular Biology Technologist (MB ASCP) with a distinguished career marked by expertise, continuous learning, and a commitment to elevating the standards of clinical laboratory science. As an ASCP Certification Expert and American Board Certification Expert, Abdelhalim Elshawadfy continues to make invaluable contributions to the field, leaving a lasting impact on the medical community.
PCR Presentation
PCR is an In vitro technique involving multiple rounds of DNA synthesis with a bacterial polymerase enzyme that is resistant to heat denaturation. A polymerase is a naturally occurring enzyme, that catalyzes the formation and repair of DNA (and RNA). Kary B. Mullis, who was awarded the 1993 Nobel Prize for chemistry for PCR. Kary Mullis conceived of a way to start and stop a polymerase’s action at specific points along a single strand of DNA.
Abdelhalim Elshawadfy is an ASCP certified Medical Laboratory Scientist (MLS ASCP), Specialist in Microbiology (SM ASCP), and Molecular Biology Technologist (MB ASCP) with a distinguished career marked by expertise, continuous learning, and a commitment to elevating the standards of clinical laboratory science. As an ASCP Certification Expert and American Board Certification Expert, Abdelhalim Elshawadfy continues to make invaluable contributions to the field, leaving a lasting impact on the medical community.
